REPORTS & DOCS

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2024

Abstract

Avian influenza (AI) is an infectious viral disease of birds, including domestic poultry, which has been causing outbreaks worldwide, leading to several millions of dead wild birds and culled poultry. AI is mainly found in birds, but recently, there was an increase in reported infections in mammals, ranging from no symptoms to mass mortality events and some human cases. Epidemiologically of great concern, evidence of mammalian adaptations have been found, but the transmission routes and pathogenesis in mammals are still to be defined. Hence, it is paramount to address all facets of AI viruses epidemiology, including investigating taxa not customarily thought to be involved in the transmission and/or trafficking of AI, such as wild mammals. The scope of this report was to assess the role of mammals in AI epidemiology, virology and pathology, i.e. AI maintenance, reservoir role, immunity, role of mammals in a potential pandemic. To do so, we performed an all-encompassing review of the literature on the topic with a two-fold approach: a systematic review of the published AI cases in wild mammals and a narrative approach to provide an expert opinion on the role of mammals in AI spread. The final number of peer-reviewed papers included in the systematic literature review was 76, resulting in 120 unique infection records with AI in wild mammal species. The most represented taxa were included in the order Carnivora. The risk of infection was identified mainly as predation (or feeding) upon infected birds or contact with avian species. Evidence of mammal-to-mammal transmission in the wild is only circumstantial and yet to be confirmed. Cases of AI from the systematic review of experimental findings were discussed concerning epidemiology, pathology and virology. Knowledge gaps and potential pandemic drivers were identified. In summary, although a greater number of infections in wild mammals have been reported, there is no hard evidence for sustained mammal-to-mammal transmission in the wild. The factors contributing to the increased number of infections found in wild carnivores are not clear yet, but the unprecedented global spread of highly pathogenic avian influenza (HPAI) viruses creates ample opportunities for intense, mostly alimentary, contact between infected wild birds and carnivores. Close surveillance of circulating strains and continued assessment of new epidemiological situations are crucial to quickly identify strains with enhanced mammalian fitness.

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Abstract

An update on the available data concerning wild boar population and ecological parameters in relation to African swine fever (ASF) epidemiology is presented. Data available on wild boar population parameters included hunting statistics collected by ENETWILD, and wild boar occurrence at high spatial resolution based on the Global Biodiversity Information Facility (GBIF). Concerning data availability on modelling the spatial distribution and abundance of wild boar, we reviewed spatial distribution models done for wild boar in Europe, indicating their main outputs and characteristics to be considered for further use in risk assessment. We also reviewed the potential use of population and risk-mediated connectivity for epidemiological studies, and their application in epidemiological studies related to wild boar and ASF; and the availability of data on scavenger communities (incl. wild boar) in Europe. Overall, recent available data on wild boar population abundance for most ASF-affected countries can be obtained from hunting statistics, which are available at high spatial resolution for some. Hunting statistics have been used to produce and validate abundance distribution models for wild boar at European level, which have been validated in mainland Europe. Recent reliable density values are scarce, but a number of them has been provided by the European Observatory of Wildlife (EOW), which offers the possibility to calibrate distribution model predictions (abundance) into densities that can be used in further ASF risk assessment. There exists an important body of literature quantifying spatial behaviour and population dynamic parameters over Europe which can be used in similar bioregions for individual/group-based models of disease spread. Important gaps on the relative contribution to scavenging of wild species remains in eastern Europe. Mainly, facultative scavengers are found in ASF affected countries, with low presence of obligate bird scavengers, and large predators at low numbers.

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Abstract

The report proposes a working plan and a strategy to carry out an updated systematic review to assess the effectiveness of various methods for separation of wild boar population in different settings. Additionally, the approach to identify and define wild boar population separation scenarios is outlined. The effectiveness of these methods based on scientific literature and unpublished field experiences is assessed, taking into consideration types of fences, different spatial and temporal features, and eco-epidemiological scenarios with a focus on African Swine Fever (ASF) in the EU. Firstly, a semi-automated scientific literature review is conducted, involving creating search strings, defining inclusion and exclusion criteria, identifying relevant databases, and analysing results using specific software packages. To supplement findings from the literature review and collect unpublished field experiences, a questionnaire is distributed to wildlife professionals through the ENETWILD consortium. This will bring an insight into unpublished experiences and provide valuable case studies for scenarios identification, bolstering the assessment of the effectiveness of methods for controlling wild boar movement. In the context of ASF spread in Europe, our conclusions represent an additional tool to draw new and effective management strategies to control ASF spread and introduction

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2023

Abstract

The European Observatory of Wildlife (EOW) as part of the ENETWILD project aims progressively developing integrated wildlife monitoring (population abundance and pathogens). The present report shows how to link the wildlife population monitoring (by camera trapping) and wildlife disease surveillance at European scale, by using environmental sampling over 15 study areas of the EOW from 10 Countries (4 study areas in 4 countries will be incorporated next). We specifically focused on multi-host pathogens Mycobacterium tuberculosis Complex (bacteria, MTC), and Hepatitis E virus (HEV). The aims of this trial were, first, to evaluate the harmonized implementation of a simple field sampling protocol for detecting zoonotic pathogens in environmental samples (standing water) through a network of wildlife professionals at European level. Secondly, we got insights for future improved strategies of wildlife integrated monitoring through environmental sampling. This trial prioritized the inclusion of a diverse array of study areas and a simple sampling approach rather than complex protocols and illustrated. We evidenced the importance of supporting such a coordinate network of wildlife professionals to progressively improve strategies, protocols, the general design, sampling, target matrix, selected pathogens, preservation and transport of samples, analytical techniques, and sample and data flow. We discuss specific results on pathogens, remarking the detection of the MTC in certain areas.

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Abstract

Wildlife policy makers and managers face challenges in taking decisions and dealing with the complexity of international context, and often operate without informed decision-making frameworks. This situation evidences the need of a harmonised Europe-wide wildlife monitoring framework able to sustain coordinated transboundary policy. With a pragmatic approach, here we intend to promote the foundations of a transnational wildlife monitoring framework in Europe, that is not meant to replace but to complement and improve harmonisation of existing monitoring plans. Here we provide a general framework on how to start up national wildlife monitoring programs to obtain comparable, aggregable results at European level. This guidance mainly deals with monitoring of species that are either abundant and managed for hunting or to prevent their impact of whatever nature, or, with species that are rare and protected, though associated with human-wildlife conflict. In the long term, rather than focusing on one single type of wildlife characteristic or monitoring component, the simultaneous monitoring of multiple components (ecological including populational, epidemiological or sociological) is an appropriate strategy to assess change and deliver integral evidence of the underlying reasons for observed changes (holistic approach). We finally raise a basic proposal indicating the main requirements to set up national wildlife monitoring programs that could be harmonisable at European level, which is based on progressive steps. The essential population and distribution data to be collected in a first instance are (i) hunting statistics, (ii) density data (relative abundance can be used for certain species and habitats) over an observatory network, and (iii) occurrence (presence/absence) data. There are different ways of integrating monitoring programs into a harmonised system. If data collected in the frame of monitoring programs are shared, ad hoc questions could be answered and coordinated wildlife management could be proactively developed, yielding reliable trends that account for factors that disregard international borders. We advocate for an integrated platform for collecting, managing, and sharing wildlife monitoring data across Europe, ensuring standardisation and consistency in the data collected by users while addressing confidentiality and secure data management.

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Abstract

ENETWILD consortium with the collaboration of the MammalNet project2 has promoted some informatic tools to improve the data collection of wildlife distribution and abundance: iMammalia; MammalWeb and Agouti. Here we update the activities in relation to (i) the new languages implemented; (ii) new functionalities, (iii) and the improvement and testing of the artificial intelligence module to identify species in Agouti. The iMammalia app is now available in 17 languages with at least two more to be added soon. MammalWeb is available in six languages with more to be added soon. Agouti is available in seven languages. iMammalia automates data transfer to the global database GBIF, and MammalWeb will consider a similar approach in the near future. Technical improvements were made to meet the needs of iMammalia as a carcass reporting app for wild boar, which will favour early awareness in case of ASF outbreak. As for density estimation through camera trapping, processing of big number of images by hand is tedious, and to facilitate the annotation process Agouti offers and has continuously improved automatic species recognition using Artificial Intelligence (AI). We summarize several topics for the further development of Agouti.

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Abstract

Expanding wild boar populations associate to conflicts with human activities, being a threat to livestock and public health. Particularly, the emergence of African Swine Fever (ASF) in Europe is of major importance. To better understanding the dynamics at the interface between wild boar and domestic pigs in Europe, which is essential to prevent the risk for ASF spread, this report describes (i) the use of extensive pig farm resources by wildlife (wild boar and other mammals) and domestic pigs, and (ii) the factors involved. We studied two regions of Central-Eastern Europe with different farm management of pigs in Serbia, in low biosecurity farms in forest/bushland habitats, and Hungary, characterized by more industrial and professional farming in fenced pastures. Camera traps (CTs) were placed at a priori risk points for interspecies interactions and in random points in 4 representative outdoor pig farms in different seasons during 2022 (2 farms in Serbia and 2 in Hungary). Also, questionnaires were distributed to 37 farms (17 in Serbia and 20 in Hungary, respectively) to describe the main features and risk factors for wildlife-pigs interaction on outdoor pig farms. CTs revealed that the wild species that more frequently used the study farms resources were golden jackal (Canis aureus) and wild boar in Serbia, and red fox (Vulpes vulpes) and red deer (Cervus elaphus) in Hungary (at the periphery of fenced farms). The use of extensive farm resources by wild boar was frequent and widespread throughout the study area of Serbia (over 33 % of daily presence per farm, 3.70 visits detected per week) whereas it was rarely detected in our sampled Hungarian farms. Wild boar visit frequency (Serbia) peaked during spring (7.5 visits per CT and day, CT*day), mainly associated with water point use (2.1 visits per CT*day). In Hungary, the greater number of direct interactions occurred between pigs and red deer during summer. Even when a higher average number of risk points were identified inside Hungarian farms, they were less permeable due to effective perimeter fencing, which prevented the entrance of wild boar and other big sized terrestrial wildlife. The study exemplifies contrasted outdoor pig farm managements, and associated risks for interaction with wild boar in ASF infected or at-risk regions. Management, characterized by almost absence of external biosecurity in the specific type of production in Serbia raise health concerns, and indicates the need to develop efforts to improve biosecurity. Several strategies and specific measures adapted to environmental conditions and farm management could reduce the interactions at the wild boar-pig interface in Serbia (and similar production systems in Eastern Europe). This should be materialized in farm-specific biosecurity programs and protocols, which requires the evaluation of their effectiveness, costs, and practical value. The type of farming practiced in marginal agricultural/forest lands in Eastern Europe, often connected to backyards production, is a highly priority for biosecurity issues at European level. However, the focus should not be only on improving technical aspects of biosecurity, but also on socio-economic and educational determinants.

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Abstract

The European Observatory of Wildlife (EOW) as part of the ENETWILD project, aims to improve the European capacity for monitoring wildlife populations, implementing international standards for data collection, providing guidance on wildlife density estimation, and finally, to promote collaborative, open data networks to develop wildlife monitoring, initially focusing on terrestrial wild mammals. This report presents density estimates for species that are widely distributed (wild boar (Sus scrofa), European roe deer (Capreolus capreolus), red deer (Cervus elaphus)) by following a standardised camera trapping (CT) protocol, in 48 areas from 28 different countries in Europe, during 2022. Density values are provided for 37 areas from 20 countries, while an additional 9 locations from 8 countries are currently completing the data analysis. The EOW involved different stakeholders over most European countries, which resulted for the first time in a number of reliable (known precision) wild ungulate density estimates, from areas representing different European bioregions. These estimates are the result of a collaborative effort from the network to apply practical systematic and rigorous protocols. The results presented from the first pilot campaign of the EOW cannot be used to accurately describe wildlife population gradients and trends at European level but can be used as first baseline data for future trend analyses. Our results show data gaps, but also provide relevant insights into some of the main drivers of demographic evolution of wild ungulate populations in Europe. We will expand and improve the EOW in the future to include more representative sites. The Agouti app, including photogrammetry methods to estimate CT detection zone size and animal speed of movement using a computer vision process proved useful to reduce the workload and to improve objectivity of measurements for REM method. We discuss the results obtained by the 2022 campaign in relation to the specific objectives of the EOW and propose the next steps.

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Abstract

The goal of this report is modelling the occurrence for carnivores at the European scale and to compare the output of occurrence with observed hunting yield (HY) density models for red fox (Vulpes vulpes) and badger (Meles meles). Random Forest function was used for modelling occurrence of species. Occurrences available from the past 30 years (1990‐2020), and HY data (period 2012‐2021) from records submitted to ENETWILD were considered for modelling. Like previous models based on HY for ungulates, the response variable was the maximum number of carnivores hunted in that period divided by the area in km2 of the corresponding administrative unit (HY density). Models based on HY were statistically downscaled to make predictions to 10×10 km2. Occurrence data models indicated a good predictive performance for most species, showing that the model framework proposed for ungulates can also be applied for carnivores. Realistic distribution maps of carnivore species were achieved under this framework, except for those ones which are expanding their range, the golden jackal (Canis aureus), or those considered alien species, raccoon (Procyon lotor) and raccoon dog (Nyctereutes procyonoides); or those having a very limited distribution as the Iberian lynx (Lynx pardinus) or the steppe polecat (Mustela eversmanii): in those cases the obtained models were underestimating their suitability in Europe. Suitability has potential to be used as a proxy for abundance of red fox and badger. Validation of suitability on HY suggested the potential to be used as a proxy for abundance of red fox and badger but depending on each species. The calibration plots for HY models showed a good and linear predictive performance for fox and badger as well as an expected pattern of abundance of species, according to the data. However, differences in type of hunting and regulations in game carnivores between countries must be playing an important role in the patterns obtained. We conclude that (i) the framework developed for modelling ungulates distribution generally well fit to carnivores species, (ii) the predicted suitability were realistic for all carnivores, but alien invasive species, limited distributed species and species expanding its range, and (iii) HY model projections displayed good abundance patterns for red fox and badger, showing that the frameworks proposed for wild ungulates were a good approximation for modelling the distribution of carnivores HY. As a future step, we need to explore how to improve the results when the unavailability of hunting activity for some species limits the extrapolation to other regions.

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Abstract

A science-based participatory process guided by EFSA identified 10 priority zoonotic pathogens for future One Health surveillance in Europe: highly pathogenic avian influenza, swine influenza, West Nile disease, tick-borne-encephalitis, echinococcosis, Crimean Congo Haemorrhagic Fever, hepatitis E, Lyme disease, Q-fever, Rift Valley fever. The main aim of this report is to formulate recommendations and technical specifications for sustainable coordinated One health surveillance for early detection of these zoonotic pathogens where wildlife is implicated. For this purpose: (i) first, we reviewed the cornerstones of integrated wildlife monitoring that are applicable to zoonotic disease surveillance in wildlife under OH surveillance in the EU; (ii) we analysed the characteristics of the main wildlife groups and the selected pathogens relevant to surveillance aimed at early detection, and integrated with other health compartments; (iii) we proposed general recommendations for the first steps of sustainable wildlife zoonotic disease surveillance in the EU, and (iv) specific recommendations of surveillance aimed at risk based early detection of pathogens in the main wild species groups. We finally proposed (iv) a framework for integrating animal disease surveillance components (wildlife, domestic, environment) for early detection under OH approach.

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2022

Abstract

The present report describes and maps the main existing structures and systematic initiatives and academic activities for surveillance in the EU for transboundary, emerging and re-emerging zoonoses in domestic animals, wildlife, and the environment, developed by the different sectors, namely human, domestic animal, wildlife and environmental, under One Health approach. This is essential to provide scientific and technical advice and improve future schemes of surveillance. A questionnaire was compiled by MSs and the information collected was complemented by literature reviews about (i) the main existing structures and systematic initiatives or activities, and (ii) academic activities for surveillance in the EU for zoonoses in domestic animals and wildlife. We focused on a 50 zoonotic diseases that were pre-selected for the prioritisation exercise by the One Health working group of EFSA. In total, 21 countries returned the questionnaire. The analysis of zoonotic disease surveillance evidenced that high fragmentation of surveillance programmes occurs in Europe and therefore the main challenge to integrate One Health surveillance is to integrate different surveillance programmes and One Health sectors to progress towards multi-host and multi-sector surveillance programmes. When different sectors oversee the coordination of surveillance programmes, the subsequent integration over the different phases of surveillance is enhanced. A structured approach is needed to determine priorities for surveillance and the approach to be used in European surveillance schemes to achieve a higher benefit-cost ratio with existing or reduced resources. The literature review indicated potential relevance of the hunting sector to participate in surveillance programmes and a bias towards research in vector-borne pathogens and vectors by the academia; experience that can be used to build One health surveillance. Recommendations are provided for further implementation of One health surveillance.

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A small proportion of disease surveillance programs target environment compartment, and in the EU these are restricted to few countries. The present report is composed of two literature reviews (i) on the main existing structures and systematic/academic initiatives for surveillance in the EU for zoonoses in the environment, and (ii) on the methods for pathogen surveillance in the environment. Concerning (i), it is noteworthy that the most frequently reported objective was to evaluate control and eradication strategies and following trends of zoonosis. However, detecting new pathogens or unusual epidemiological events were scarcely reported as objectives, as well as demonstrating freedom from a particular pathogen, despite the big potential that environmental sampling and testing techniques have recently demonstrated for these purposes. Few of the pathogens prioritised by EFSA were represented in this literature review, indicating the potential of environmental techniques to be applied to a larger extent to detect relevant transboundary and (re)emergent zoonoses. The preferred environmental sample was water, followed by biological material (included faecal material) and vectors (mosquitoes). To a much lesser extent, soil, and other matrices were used. Regarding (ii) the pathogen detection and identification methods were divided into: conventional (culture and biochemistry-based, and immunology-based); molecular methods (nucleic acid-based methods); biosensor-based (new) and others. A large percentage of available assays for the detection and surveillance of pathogens in the environment focuses on hazards that are not among those pre-selected by EFSA. Therefore, there is a need for development of new, untested, methods for surveillance of listed pathogens of higher epidemiological importance. Less disturbed areas, natural and wild environments are less covered by environmental sampling techniques than urban and farm environments and should therefore receive higher attention since they may hold undiscovered and potentially epidemiologically significant hazards and hosts. In general, molecular methods, namely the nucleic-acid based methods, are the ones more commonly and widely used for pathogen detection in environmental samples, and can be developed for virtually any organism, given a sufficient effort to identify specific DNA/RNA sequences unique to the target organism. The usefulness and appropriateness of different environmental matrices for detecting specific pathogens or for specific purposes are discussed and recommendations are provided.

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Abstract

EFSA has been asked by the EU Commission to assess the prioritization of cross-border pathogens that threaten the Union to be used in setting up a coordinated surveillance system under the One Health approach. A list of 50 pathogen has been considered in a first stage by the Working Group of EFSA. Under this approach, the criteria to be applied for prioritization of pathogens in further steps should consider that emerging infectious diseases have arisen from or been identified in wildlife, with health implications for humans, domestic animals, and wildlife. The identified infectious diseases pose a threat to wildlife populations and biodiversity and are perceived by the society as real risks to wildlife conservation. Here we review the endangered wildlife hosts in Europe that may be affected by the selected pathogens. We elaborated a list of potential endangered wildlife hosts distributed in Europe for each pathogen, which were sortied following their taxonomic classification. Hosts species were classified (as a function of their conservation status) as Near Threatened (NT), Vulnerable (BVU), Endangered (EN) and Critically Endangered (CE) based on the International Union for Conservation of Nature’s (IUCN) Red List of Threatened Species. Their endemicity status (to EU and to Europe, respectively) was also indicated. To consider a species as potential hosts, a literature review was performed. We detailed the taxonomic level at which each pathogen has been reported (from Order decreasingly to Species) so as the reporting of clinical signs, paying attention also to reports in the wild, but also in zoological collections. A complete table and data sources are presented. Pathogen specific cards to illustrate the main findings in relation to threatened wildlife host species are disseminated to experts on different disciplines to raise awareness about the relevance of wildlife conservation under the One Health approach and to promote the integration of this approach into management of human-wildlife conflicts and conservation.

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Surveillance systems for zoonotic and transboundary emerging pathogens that are structured following the holistic principles of joint work efforts from the human health, animal health and environmental health sectors are reviewed to provide a summary of one-health based surveillance systems existing worldwide. A systematic search of available literature was undertaken across various biomedical and scientific literature databases (from 2000 to 2022) and were selected using inclusion/exclusion criteria to filter references presenting systematic surveillance systems applicable to transmissible, transboundary, and zoonotic diseases operating under the One Health approach. A standardized data model and vocabulary were used to extract and classify key information to characterize target surveillance systems. 996 studies were obtained after research (589 after duplicates’ elimination) for inclusion in this review and information was extracted using a data model, which were reduced to 79 items once inclusion and exclusion criteria were applied. From these articles, 80 additional items were found within references. West Nile virus, followed by rabies and Rickettsiae are the main target pathogens for which One Health efforts are in place within structured and systematic surveillance systems. The worldwide revision of surveillance systems for emerging and transboundary zoonotic diseases evidenced specific targets for one-health efforts which differ from the targets of sectoral surveillance and are specifically prone to a cooperative approach.

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Abstract

The International Symposium on Wild Boar and Other Suids (IWBS 2022), which took place in Montseny Biosphere Reserve (Catalonia, Spain) in September 2022, provided to ENETWILD with the opportunity to meet in-person for the first time after 2.5 years, and meet the international scientific community with expertise on wild suids and other ungulates. Twelve members of ENETWILD consortium representing 6 partners were present. Bringing together international experts, stakeholders and ENETWILD collaborators was a perfect occasion to present the European Observatory of Wildlife (EOW). Two hundred and twenty-five wildlife experts from 25 countries were present at symposium, and at presentation of the EOW. Overall, 3 ‘Plenary Talks’ and 118 presentations (62 oral and 56 posters) were made. The meeting has gone through all the possible topics regarding wild suids, from genetics to monitoring and management. This was the optimal context to introduce the EOW to an ideal target audience, both in terms of interest and in terms of potential new member of the Network. From our presentation, it emerged the importance of comparable data on geographical distribution and abundance of wildlife hosts in Europe, fundamental to develop the best management policies and to perform effective risk assessments for shared emergent diseases. The adoption of a common and effective protocol adopted throughout the continent would ensure such comparability. Moreover, the discussion highlighted the need of extending the network to as many European countries as possible and, when feasible, of having multiple sites within each country. A number of participants manifested their interest to join the EOW during the 2023 campaign. Such a capillary distribution of observation points would provide solid and comparable density estimates as well as effective feedback about the field protocol implemented by the EOW. A number of questions were raised by the audience during the presentation of the EOW.

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Abstract

Wildlife diseases have an impact on biodiversity, economy, and public health. As this impact increases with climate change, land‐use change and trade, a multidisciplinary approach in analysing data from these different disciplines is increasingly required. The need for a common data standard to improve data sharing of surveillance efforts is thus larger than ever to enable transnational proactive and reactive measures to be taken. The Enetwild consortium, funded by the European Food and Safety Authority (EFSA), evaluated the capacity for the Darwin Core standard to handle wildlife disease data. In addition to two new terms previously proposed by Enetwild, the Darwin Core standard proved to have the flexibility to handle complex datasets from a wide variety of research and surveillance sources from local to international scales. Integration of the data model to the Global Biodiversity Information Facility is discussed, and controlled vocabularies specific to epidemiological data are proposed.

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Abstract

The goal of this report is i) to model the occurrence and hunting yield (HY) density of wild ungulates not only for widely distributed species in Europe, but also for those ones which have a constrained distribution and ii) to compare the output of occurrence with observed HY. Random Forest function was used for modelling occurrence of species. We used occurrence data available from the past 30 years, and HY data (period 2015-2020) from records collected by ENETWILD. Like previous models based on HY, the response variable was the maximum number of wild ruminants annually hunted in 2015-2020 hunting seasons divided by the area (km2) of the corresponding administrative unit (HY density). Models based on HY were statistically downscaled to make predictions to 10x10km squares. Occurrence data models indicated a good predictive performance for most species, showing that the model framework proposed have improved results in comparison to previous models. The transferability of models into new regions was limited by the exposure of species to environmental conditions. As for HY models, the calibration plots showed a good and linear predictive performance for widely distributed species, as well as constrained distributed species. Overall, our results were consistent with the expected abundance distribution of widely distributed species. The removal of zeros on the validation datasets affected the calibration plots of all regions, showing a better predictive performance when zeros were removed for widely distribution species, but the opposite was evidenced for species with limited distributions. We conclude that (i) the importance of co-correlation variables when variable importance is inferenced from random forest model results, (ii) manipulation presence and absence locations could yield further improvement in occurrence model outputs, and (iii) HY model projections displayed good abundance patterns for most of species, showing that the three frameworks proposed were a good approximation for modelling the distribution of wild ungulates HY, although it should be explored how to improve the results when distribution is patchy.

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Abstract

The EU‐Commission is setting up a coordinated surveillance system under the One Health approach for cross‐border pathogens that threaten the Union, for which EFSA is assessing the prioritization of pathogens to be targeted by surveillance. To support in prioritizing pathogens, this report reviews the literature on existing frameworks, describes the criteria to be considered for prioritization and compares approaches used in the reviewed studies (from year 2000 onwards). The search was undertaken across various biomedical and scientific literature databases and were selected using inclusion criteria to filter references presenting prioritization criteria/tool/methods applicable to transmissible and zoonotic diseases. A data standardised model was used to extract key information to characterise disease prioritization frameworks. One‐thousand one‐hundred and thirty‐eight studies were selected for inclusion in this review, which were reduced to 80 items once the inclusion and exclusion criteria had been applied, for which, statistics are presented. Most of these studies used one of six methodologies to prioritise disease risks: bibliometric index, the Delphi technique, multi‐criteria decision analysis (MCDA), qualitative algorithms, questionnaires, and multi‐dimensional matrix. Overall, the review of referenced papers indicated that, regardless of the selected method, (i) it is essential that when using experts the criteria reflects the aims of the risk‐ranking exercise, (ii) a large and multi‐disciplinary panel can further mitigate subjectivity and professional bias, (iii) all relevant stakeholders should be included in the process, (iv) weighing of criteria to rank pathogens should ideally be done at a separate time or by a separate group to reduce bias, and (v) it should be evaluated from the very beginning if the project team has the necessary expertise or if outsourcing is required for a given method. Indications are given for the methods to prioritise pathogens, remarking that, for a comprehensive risk ranking including novel, emerging and established infections, ECDC recommends MCDA or Delphi methods (which are here descriptively compared), which are comprehensive methods for risk ranking. We recommend a further detailed evaluation (as recommended by ECDC) of listed references based on their validity and reliability and including grey literature.

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Abstract

In a previous report, ENETWILD proposed a generic model framework to predict habitat suitability and likely occurrence for wild ruminant species using opportunistic presence data (occurrence records for wild ungulate species from the Global Biodiversity Information Facility). In this report, for the first time, we develop models based on hunting yield data (HY) for the most widely distributed wild ruminant species in Europe: roe deer (Capreolus capreolus) and red deer (Cervus elaphus). We also update models based on occurrence (roe deer, red deer, fallow deer (Dama dama), European moose (Alces alces) and muntjac (Muntiacus reevesi), evaluate the performance of both approaches, and compare outputs. As for HY models, we could not conduct one model per bioregion as there are not enough data for modelling in some bioregions, and therefore, we calibrated a unique model, including eco-geographical variables as predictors. The calibration plots for HY models showed a good predictive performance for red deer in the Eastern bioregion and roe deer at Eastern and Western. The abundance distribution pattern of red deer HY was widely scattered over all Europe, as expected for a widely distributed species which shows high ecological plasticity, and roe deer presented the highest abundance in Atlantic and Eastern Europe, progressively decreasing towards Northern Mediterranean bioregions. Overall, calibration plot did not perform well in the Northern region, which could be due to the low availability of data for both species in this bioregion. As for occurrence data models, performances using our revised approach for most species showed similarly moderate predictive accuracy. To sum, HY model projections showed good patterns where good quality data was provided, while worst predictions are found in neighbouring countries/bioregions. Two approximations to be explored for next models are: (i) modelling HY per bioregion providing more flexibility to the models, even if data projection is done at lower resolution scales, and (ii), modelling HY by accounting the fact that certain countries provide most data, to avoid that these areas overinform the model. As for occurrence data model, next steps for data acquisition and occurrence data modelling are: (i) review target group definitions for each species, (ii) revise definitions of “true” absence for model testing for better parity with fitting, and (iii) either replace principal component analysis with variance inflation factor analysis to remove co-correlates and model calibration for variable selection or develop post-model analysis to recover environmental dependencies.

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Abstract

Camera traps (CT) provide an easy and non-invasive way to study wildlife. It is also possible to estimate densities if accurate protocols are followed in a standardised way and additional parameters are estimated. However, the processing and storage of the thousands of images that a typical CT study generates has become a major challenge for CT users. Also, project management can get complex, especially if many CT and multiple people are involved. To facilitate collaborative science among professionals and semi-professionals, the ENETWILD consortium is developing the existing Agouti platform for the management of camera-trapping projects, and the processing and storage of images. Moreover, the consortium is extending Agouti with tools for doing the measurements needed for acquiring the additional estimates and is building an R package to estimate actual density. These developments will significantly further the completion of the Agouti ecosystem. A network of CT-based abundance estimations is consolidated by providing analytical tools and by promoting collaborative science. Specifically, we have worked to (1) harmonize dataset generation by means of Agouti and (2) develop an interface for running CT abundance models (REM, REST, distance sampling). After completion of this work, users should be able to easily export their camera trap records into a format (camtrap-dp) that can subsequently be used to easily run models and determine density using an interface in R, following the methods recommended by ENETWILD. The progress that has been made to date in relation to data generation and analysis is detailed; interactive maps and institutional portals; data recording for abundance estimation; distance and speed estimation, making distances part of camtrap-dp; and finally, R package for distance, speed, and density calculation.

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Abstract

Citizen science (CS) has been increasingly used in wildlife disease surveillance since it can facilitate the detection and recovery of carcasses of target species used as sentinels of infection. This report updates on the improvement of an app adapted from iMammalia (MammalNet project) for early reporting carcasses of wild boar with a warning system. This app has a great potential for improving wildlife disease surveillance (carcass reporting), such as for ASF. The app has been expanded in the context of ENETWILD project during the second part of 2021 to several countries in their respective languages: Italy, Greece, Serbia, Montenegro, Kosovo, Bosnia-Herzegovina, North Macedonia, Albania, and the latest to incorporate, Portugal. The ENETWILD project has generated alliance with FAO to promote the use of iMammalia in the Balkans to collect data in gap areas about the distribution of wild boar, to document the presence of carcasses of dead animals. The technical improvements that have been made to meet the needs of iMammalia to report the presence of wild boar carcasses correspond to the possibility of recording different parameters, such as sex, age, whether the dead animal has been hunted or not, or the degree of decomposition observed. The possibility of generating warnings to notify these records in real time to wildlife and/or sanitary services will be implemented in 2022. To date, iMammalia collected 14,393 mammal observations all over Europe, 797 of which corresponds to wild boar. A total of 1,270 observations correspond to animals found dead (5.53%), 30 out of which are wild boar, which, in some cases, were later diagnosed as ASF positive (e.g. in Serbia). We finally summarize the next step of ENETWILD in relation to iMammalia promotion, including an online workshop to provide an overview of disease surveillance systems in Europe, and to propose changes, including the promotion of CS.

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Abstract

The definition of the most relevant parameters that describe the wild boar (WB) population dynamics is essential to guide African swine fever (ASF) control policies. These parameters should be framed considering different contexts, such as geographic, ecological and management contexts, and gaps of data useful for the parameter definition should be identified. This information would allow better harmonized monitoring of WB populations and higher impact of ASF management actions, as well as better parametrizing population dynamics and epidemiological models, which is key to develop more efficient cost-benefit strategies. This report presents a comprehensive compilation and description of parameters of WB population dynamics, including general drivers, population demography, mortality, reproduction, and spatial behaviour. Beyond the collection of current available data, we provided an open data model to allow academics and wildlife professionals to continuously update new and otherwise hardly accessible data, e.g. those from grey literature which is often not publicly available or only in local languages. This data model, conceived as an open resource and collaborative approach, will be incorporated in the European Observatory of Wildlife (EOW) platform, and include all drivers and population parameters that should be specified in studies on wild boar, and wildlife in general, ecology and epidemiology at the most suitable spatio-temporal resolution. This harmonized approach should be extended to other taxa in the future as an essential tool to improve European capacities to monitor, to produce risk assessment and to manage wildlife under an international perspective.

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Abstract

The new‐born European Observatory of Wildlife (EOW)2 is a part of the EFSA‐funded ENETWILD project, and has the aim of improving the European capacities for monitoring wildlife populations, implementing international standards for data collection, providing guidance on wildlife density estimation, and finally, to promote collaborative, open data networks to develop wildlife monitoring. As a next step, the EOW has engaged and enhanced the existing network of collaborators, and a number of participants are currently preparing field operations to estimate wild mammal density (focused on wild ungulates and other medium to big sized mammals) in certain areas from their respective countries. A field camera trap (CT) based protocol provided by the EOW is going to be applied. An online training course held in May 2022 provided specific training on camera trapping methods and protocols, specifically the random encounter method (REM) and other methods which do not require individual recognition. Here we also present the new field protocol, which is compatible with the subsequent application of artificial intelligence to process and analyze photo trappings using the online app AGOUTI. This strategy aims at promoting a network of professionals/researchers capable of designing, developing field work and analysing data, contributing also to disseminate the experience and train other colleagues in their respective countries. By now, the overall number of countries participating in the EOW is 25. Some participants from 12 countries could already estimate mammal densities during the previous seasons 2019/2020/2021, which will also apply the same methodology in different populations during 2022 in their respective countries. The number of density values finally obtained through this experience by the end of 2022 will exceed 40 different locations in a total of at least 30 countries, since some countries are on the process to confirm their participation. The EOW website is presented. This coordinated field trial activity over a range of European countries, involving different experts and professionals, follows the original plan.

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Abstract

This report presents the results of field activities in relation to the generation of reliable wild boar density values by camera trapping (CT) in 19 areas in Europe, mainly in East Europe. Random Encounter Model (REM) densities ranged from 0.35±0.24 to 15.25±2.41 (SE) individuals/km2. No statistical differences in density among bioregions were found. The number of contacts was the component of the trapping rate that determined the coefficient of variation (CV) the most. The daily range (DR) significantly varied as a function of management; the higher values were detected in hunting grounds compared to protected areas, indicating that movement parameters are population specific, and confirming the potential role of hunting activities in increasing wild boar movement and contact rates among individual or groups. The results presented in this report illustrate that a harmonized approach to actual wildlife density estimation (namely for terrestrial mammals) is possible at a European scale, sharing the same protocols, collaboratively designing the study, processing, and analysing the data. This report adds reliable wild boar density values that have the potential to be used for wild boar abundance spatial modelling, both directly or to calibrate outputs of model based on abundance (such as hunting bags) or occurrence data. Future REM developments should focus on improving the precision of estimates (probably through increased survey effort). Next steps require an exhaustive and representative design of a monitoring network to estimate reliable trends of wild boar populations as a function of different factors in Europe. In this regard, the newly created European Observatory of Wildlife will be a network of observation points provided by collaborators from all European countries capable to monitor wildlife population at European level.

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Abstract

One of the main objectives of ENETWILD consortium is to collect data on density, hunting statistics and wildlife occurrence in order to model the geographical distribution and abundance of wildlife species across Europe as a tool to support the assessment of risks associated, for example, with disease transmission. Created in the framework of the ENETwild project, the European Wildlife Observatory (EOW2) provides the backbone for an integrated, interdisciplinary, multi-sectoral and multi-institutional approach to wildlife monitoring, initially focusing on terrestrial mammals in Europe. The EOW applies similar camera-trapping-based protocols for population estimation and data collection standards to facilitate harmonization and interoperability. For this purpose, continuous training of the network of wildlife professionals in Europe is a key activity of the EOW. In this context, during the last few years the ENETWILD consortium has organized different online training courses and workshops on the use of camera traps, addressing different approaches from the design and handling of camera traps to the processing of the collected data. Many of the participants in our previous courses are now part of the EOW and require updated information on methodology to process with next steps in the field. The course here reported presented improvements and refinements in the sampling protocols, aimed specially at new collaborators to be incorporated in the network. Therefore, the objectives of this introductory online course held on 5th May 2022 were: (i) to present milestones and achievements of the ENETWILD project and the EOW, and (ii) to review scientific methods for determining wildlife abundance and density, providing specific training on camera trapping methods and protocols, specifically the random encounter method (REM) and other methods which do not require identification of individuals. This course was attended by 46 wildlife biologists, animal health professionals and wildlife experts from national hunting and forestry authorities. Detailed explanations, protocols, and examples for applying such protocols were provided.

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2021

Abstract

ENETWILD consortium has developed methodologies for modelling wild boar abundance distribution based on hunting yield (HY) data. Although the methodologies reached an acceptable reliability, when models were downscaled to higher spatial resolution the predictions of absolute numbers of hunted animals tended to overprediction. Some important issues such as HY‐surface relationship and the spatial autocorrelation of HY data or the accuracy of downscaled predictions were not fully addressed yet due to the complexity of dealing with huge datasets at a European scale. In this report we (i) explored the use of hunted wild boar densities (numbers of hunted wild boar relative to surface) instead of raw counts (numbers of hunted animals) as response variable, and (ii) introduced intrinsic Conditional Auto‐Regressive models (iCAR) taking into account spatial autocorrelation. Using simulations and actual wild boar data, these new actions were aimed to produce high resolution predictions (2×2 km grid) with higher accuracy. We assessed model fitting in two different regions in Europe with high quality resolution HY data: Aragón autonomous region (North East Spain, belonging to South Bioregion as defined by ENETWILD) and the whole country of Slovenia (East Bioregion). We found that the marked overprediction, as observed in previous reports when models were downscaled, was now controlled by using hunted wild boar densities as response variable. Additionally, higher accuracy in model predictions was reached when iCAR approach was used to control for spatial autocorrelation. This high accuracy was maintained even when high resolution predictions were aggregated and compared to actual wild boar HY. These approaches should be considered in future models and represent an important step forward to model the distribution of wild boar abundance and other wildlife at high resolution over Europe.

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Abstract

In order to define the spatial interface between wild boar and domestic pigs in Europe, the ENETWILD consortium (www.enetwild.com) described in a preliminary report the different sources of data for domestic pigs at European scale, and developed a preliminary risk map of possible spatial interaction between both groups. This model explored and assessed the use of pig distribution data from Gridded Livestock of the Worlddatabase (GLW), FAO. However, in some specific countries used as cases, the GLW predictions did not reliably represent the pig abundance distribution within countries. The currently available census data of livestock at the European Union level (Eurostat) is limited to the spatial resolution at NUTS2. While Eurostat ensures that data can be potentially comparable,there is still needed to resolve definition issues regarding better spatial resolution (level of aggregation of information) and the pig production systems. In this context, the objectives of this report are (i) assessing the spatial interface between pigs and wild boar over Europe using the best quality data available (Eurostat data and ENETWILD spatial models). We(ii) secondly assessed the interface at higher spatial resolution, distinguishing pig production types in countries where data was available. Based on comparisons at different scales and quality of data, we propose future steps in both data collection and modelling approach.Precisespatial resolution of pig data is not available at European level yet, and the discrimination of extensive vs. intensive farms, backyards vs. commercial; outdoor vs. indoor, is essential to quantify and perform risk analyses separately for each production system and/or considering this relevant source of variation in risk at the interface. The development of a framework to collect harmonised and standardised data at European scale at higher resolution is needed.

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Abstract

The ecological plasticity of wild boar and their growing populations can generate conflicts with human activities and can be a threat to livestock and public health. Particularly, the emergence of African Swine Fever in Europe is of major importance. However, there are gaps in knowledge about wild boar ecology, population monitoring, management and population control that prevent the design and application of the best science-based ASF control policies, and/or adaptive evaluation of the actions taken. The effectiveness of wildlife policies is known to be directly proportional to their acceptance by stakeholders. However, it is unknown how the acceptance of these policies and different management scenarios vary among stakeholder groups, in different socio-economic and cultural contexts. Acceptance by stakeholders in different contexts determines the success of management strategies. Finally, factors that influence wild boar abundance and disease spread are not bound by national borders. Thus, there is need to coordinate national and international decision-making. In this context, this report presents research protocols to address a number of knowledge gaps previously identified by EFSA, and aims to improve the strategy to control ASF in the short-term. Twelve research objectives grouped into six categories address aspects of: (i) wild boar ecology, i.e. studies on basic aspects of wild boar population dynamics and assessment of the factors that determine the presence of wild boar near outdoor pig farms; (ii) wild boar monitoring, i.e. implementation of practical methods to estimate wild boar density and strategies to promote their application; (iii) wild boar management and population control, i.e. effect of feed availability, role and efficacy of recreational hunting and professional culling, efficacy of wild boar trapping and different fencing methods and the use of trained dogs in ASF affected areas; (iv) social acceptance by the stakeholders; (v) assessment and management of risk factors (biosecurity awareness and implementation among backyard pig farmers, evaluation of passive surveillance and carcass removal); and (vi) national and international decision-taking. We propose protocols for each specific research objective, their study design, implementation methodology, required time frames and budget limitations. We comparatively summarize the protocols and discuss them in terms of solving overlaps and interactions among protocols that address different research objectives, which eventually can be combined to optimize the use of resources and budgets and to reduce the required time needed to achieve objectives.

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Abstract

The activities related to the generation of reliable wild boar density values in at least 15 areas in Europe by camera trapping, mainly in East Europe are presented. The ENETWILD consortium has offered training to collaborators in order to generate harmonized wild boar abundance data (following standards) and has enhanced the network of wildlife professionals in Europe. As a next step, ENETWILD has engaged and enhanced this network, and a number of participants are currently developing field operations to estimate wild boar density in certain areas from their respective countries or confirmed that will do during summer 2021. A protocol for field camera trap (CT) provided by ENETWILD is being applied. By now, 12 field studies are ongoing in 10 countries and in most of them data has been already collected. In addition, there are 3 countries (Albania, North Macedonia and Turkey) in which, due to logistic problems during transport of the CTs at customs, the field work will be developed during 2021. In April 2021, ENETWILD is offering a second online training course to participants, so they will be trained specifically in data analysis. This strategy aims promoting a network of professionals/researchers capable of designing, developing field work and analysing data on their own, contributing also to disseminate the experience and train other colleagues in their respective countries. Most participants, who were already estimating wild boar densities during the season 2019/2020, will also apply the same methodology in different areas during 2021 in their respective countries.

Therefore, the number of wild boar density values finally obtained through this experience by the end of 2021 will exceed 15 different locations in a total of 12 countries.

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Abstract

The main objective of ENETWILD is to collect data on wildlife density, hunting and occurrence and to model geographical distribution and abundance of wildlife species throughout Europe. This subject is of particular concern in the case of wild boar due to the continued advance of African swine fever (ASF). Training, generation of harmonized wildlife abundance data following defined standards and enhancing the network of wildlife professionals in Europe is a key activity of the project that, especially in previously identified gap areas (eastern Europe). In this context, the ENETWILD consortium previously organised an online training course on camera trapping in September 2020. An outcome of this previous course was the need to organizing specific and more intensive training focused exclusively on camera trap data analysis. Therefore, the objectives of a second online course carried out in April 2021 were: (i) to be able to prepare a datasheet (using their own data or those provided by ENETWILD), and (ii) to analyse the data to estimate the density and error intervals. This course was attended by 53 game biologists, animal health professional and wildlife experts from national hunting and forest authorities. Detailed explanations, protocols and examples to implement such analyses were provided. This course on the use of camera trapping for monitoring wildlife and density was useful to complete training of a network of collaborators which are estimating wild boar densities over gap regions of Europe and are funded and supported by ENETWILD. They are now self-sufficient to apply field protocols and to analyse data. In addition, several participants manifested their interest to join this initiative by using their own means, contributing to this pioneer network of harmonized wildlife monitoring over Europe

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Abstract

In the previous ENETWILD model, the predicted patterns of wild boar abundance based on hunting yield data reached an acceptable reliability when the model was downscaled to higher spatial resolution. This new approach, based on the modelling of hunting yield densities instead of hunting yield counts and the assessment of spatial autocorrelation, was only applied with simulated data and with data from two regions at hunting ground level, the smallest spatial resolution. In this report, (1) we evaluate whether this approach can correct the overpredictions for high‐resolution predicted patterns when raw data are present at a different spatial resolution (i.e. the European region). For this purpose, hunting yield densities were incorporated as response variable (one model per bioregion) and predictions reliability at 10x10km and 2x2km spatial resolution were assessed. Internal validations and comparisons with the previous two‐step model carried out at European scale were addressed, as well as an evaluation with external data at the same scale at country level. The model presented certain overprediction (much less than the previous model) of the total hunting bags reported per country, although a good correlation in terms of values and linearity between observed and predicted values was achieved. Secondly (2), a generic model framework to predict habitat suitability and likely occurrence for wildlife species using opportunistic presence data was proposed (occurrence records for wild ungulate species from the past 20 years exclusively from the Global Biodiversity Information Facility extracted on 9/12/2020). Across all wild ungulate species (elk (Alces alces), roe deer (Capreolus capreolus), red deer (Cervus elaphus), dam deer (Dama dama), muntjac (Muntiacus reevesi), wild boar (Sus scrofa)) the model framework performs well. For those species where area under the curve is below 0.7 we note lower accuracy in predicting absences, which requires further investigation to understand the root cause; whether a result of underlying assumptions regarding the testing data or due to the model performance itself.

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Abstract

The 2nd ENETWILD Annual General Meeting took place on 5-6th October 2021, bringing together experts, stakeholders and ENETWILD collaborators in online workshop discussions. First, workshop discussions contributed to the analysis and proposal of approaches for a harmonized European-wide wildlife monitoring framework able of sustaining coordinated decision-making. Secondly, participants identified the key challenges that managers face in making decisions for wildlife in Europe and data needs for policies. Finally, we illustrated these challenges with the case of wild boar as a model species widely distributed across Europe. Inputs from the participants were collated into a plan of proposed steps and objectives for the mid-term (5-year time frame) to achieve progress on harmonised, coordinated, and integrated wildlife monitoring at the European level, which requires the contribution of experts from the early stages.. Specific proposed actions include the creation of a trans-disciplinary authority at the European level, effective points of reference for data collection and sharing at different administrative levels and countries, a standing committee to coordinate and exchange experience and capacities on data collection between countries, and expert groups for problem solving, with proper EU financial support, establishing regular policy meetings. . To provide useful results, wildlife monitoring must ensure proper design and data analysis for subsequent science-based management and best allocation of management resources. The ‘Observatory’ approach (a representative network of intensively monitored sites) can provide long-term systematic and representative insights, normally more feasible for comparative studies, providing less biases and support for decision-making. For international decision-making by wildlife managers and politicians based on scientific knowledge and interdisciplinary research, experts should define the foundations of a common European wildlife decision-making framework (inter-institutional and inter-sectorial). The development of a European legislation on wildlife management may represent an opportunity for addressing the abovementioned steps, identifying data priorities matching the needs of the various European Directorates, Agencies, and monitoring frameworks.

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Abstract

The ENETWILD consortium (www.enetwild.com) aims at progressively defining the spatial interface between wild ungulates and livestock in Europe, which is essential to evaluate the risk for shared diseases. This is to provide preliminary risk maps of possible wild-domestic interfaces at European scale using relatively similar sized regions by compiling, for the first time, comprehensive data for both groups, wild and domestic ungulates in the continent. We spatially represented (i) the richness of species (livestock and wild ungulates), (ii) their specific occupancy and abundance (the latter for livestock), and finally, (iii) their spatial overlapping over Europe. Species richness in animal communities, including wildlife and domestic hosts, may moderate pathogen transmission and disease outcome.. As a first step, we should characterize the diverse assemblages of animal communities at large scale to better understand possible scenarios for further assessment of shared infection dynamics. About 90% of Europe land area hosts from one to five species of wild native ungulates. Therefore, the interface between livestock and wildlife is wide spread over the European continent. Native wild boar, roe deer and reed deer are widely distributed species, present in most possible assemblages of wild/domestic communities over Europe. The richness of ungulate species is high in Central Europe, from West to East, from the Alps (where the presence of mountain ungulates adds richness), extending to countries with important big game tradition and presence of introduced species, and finally, to Eastern Europe (where also typically northern species such as bisons appear)… To sum, we described by pair of species a wide diversity of potential interfaces, which had variable distribution areas.. While the analysis presented herein is purely spatial and at administrative level, the interface between wild and domestic ungulates is influenced by livestock husbandry (e.g., enclosed, herded or free-ranging, level of biosecurity), landscape and land uses, and wildlife management practices, among other factors, operating locally. Therefore, there is need for a more detailed picture of the interface at European scale.

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Abstract

The activities related to the generation of reliable wild boar density values in at least 15 areas in Europe by camera trapping, mainly in East Europe are presented. The ENETWILD consortium has offered training to collaborators in order to generate harmonized wild boar abundance data (following standards) and has enhanced the network of wildlife professionals in Europe. As a next step, ENETWILD has engaged and enhanced this network, and a number of participants are currently developing field operations to estimate wild boar density in certain areas from their respective countries or confirmed that will do during summer 2021. A protocol for field camera trap (CT) provided by ENETWILD is being applied. By now, 12 field studies are ongoing in 10 countries and in most of them data has been already collected. In addition, there are 3 countries (Albania, North Macedonia and Turkey) in which, due to logistic problems during transport of the CTs at customs, the field work will be developed during 2021. In April 2021, ENETWILD is offering a second online training course to participants, so they will be trained specifically in data analysis. This strategy aims promoting a network of professionals/researchers capable of designing, developing field work and analysing data on their own, contributing also to disseminate the experience and train other colleagues in their respective countries. Most participants, who were already estimating wild boar densities during the season 2019/2020, will also apply the same methodology in different areas during 2021 in their respective countries. Therefore, the number of wild boar density values finally obtained through this experience by the end of 2021 will exceed 15 different locations in a total of 12 countries.

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2020

 

Abstract

The ENETWILD consortium (www.enetwild.com) aims at defining the spatial interface between wild boar and domestic pigs in Europe, which is essential to evaluate the risk for ASF spread between wild and domestic. This report describes the different sources of data for domestic pigs in Europe and develops a preliminary risk map of possible spatial interaction between both groups. Specific cases from Romania and Spain where reliable data were available were assessed. This modelof the interface was based on the data from Gridded Livestock of the World(GLW) database of FAO, which provides predictions on a 1×1 km scale globally and the wild boar abundance distribution model recently elaborated by ENETWILD consortium (2020).The present available census data of livestock at the European Union level (Eurostat) is restricted to a maximum spatial resolution of NUTS2, remarking the need of developing a framework to collect harmonised data with higher resolution. This will ensure that data can be comparable, validated and used. There is need also to resolve definition issues regarding the pig production systems. Our prediction model of the interface between pig and wild boar at European level indicated that the maximum risk is scattered over Central Europe, large parts of Spain, north‐east France and Romania. Hungary, so as Serbia and Croatia in the Balkans are at the highest risk in that area. In the specific case of Romania, no statistically significant association between census data of pigs collected fromnational authorities and predicted values was found when assessing the GLW model, evidencing that GLW predictions do not reliably represents the pig abundance distribution within countries. When assessing the interface model in Spain, certain areas of interaction were lacked, e.g. where extensive farming is relevant. The current discrimination of extensive vs. intensive farms of predictive models (GLW) is not reliable to perform analyses separately for each production system.The outputs this model of interface between wild boar and domestic pigs will guide future steps in both data collection and modelling approach.

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Abstract

Enetwild consortium aims at aggregating data on occurrence, abundance and hunting bag of wildlife in Europe, either as raw data or as results of statistical estimation. These data come from a large community of researchers, hunters and wildlife managers. A flexible and robust data standard is therefore necessary to present the large diversity of data and collection method. We evaluated the possibilities offered by the Darwin Core Standard. The Event core, the occurrence extension and the extended measurement or fact extension proved their utility for our purpose. However, these were not able to record statistical estimation values. We proposed to extend the measurement or fact extension to allow them to be nested among themselves. Any confidence interval or precision measure is indeed a measurement about the punctual estimate, another measurement. We proposed controlled vocabularies adapted to wildlife survey in data and metadata. This will be aligned with the EFSA data model harmonisation under the SIGMA project.

 

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Abstract

The ENETWILD consortium updated in August 2019 suitability maps of wild boar occurrence and relative abundance based on hunting statistics, providing predictions at 10×10 km. External validation of this relative abundance model and a new downscaling on a 2×2 km grid was addressed in the ENETWILD report in January 2020. In this report, we update and incorporate additional data to provide new maps of wild boar suitability with a resolution of 2×2 km, obtained from a presence‐only algorithm, and a new model based on hunting yield data for MS and neighbouring countries. Hunting yield‐based modelling provides further novelties in two ways: new predictor variables and new (two) modelling approaches, i.e. smooth bioregion modelling and two‐step independent bioregion modelling). Internal validations and comparisons among previous and new suitability and hunting yield models were also addressed, as well as external evaluation of the best new approach at European scale (at country level). The suitability map showed a good agreement with the expert‐derived species range published by the International Union for Conservation of Nature. New models of relative abundance performed in general better than the previous one according to internal validations, concretely the two‐step independent bioregion approach gives the best validation scores. This approach solved the abrupt changes in predictions between bioregion boundaries. As with the previous model, the external evaluation of the new model based on hunting yield presented certain over‐prediction of the total hunting bags reported per country, although a high linearity between observed and predicted values was achieved. Previous and new hunting yield model predictions showed disagreements particularly in North and East regions, and other scattered areas in South and West, being areas in which the new model provides more reliable predictions. Hunting yield model outputs showed a relevant improvement in smoothing transitions between bioregions due to the flexibility provided by the new approach. Our analysis showed only partial agreement between suitability and relative abundance models, and reasons for these differences are discussed in this report.

 

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Abstract

The methods for estimating relative abundance and density in wild ruminant species are reviewed and insights on how to obtain reliable estimations by using those methods are provided. Eighteen methods used in nineteen wild ruminant species widely distributed across Europe are reviewed. In accordance with the ENETWILD consortium objectives, we evaluate if different types of data can be used to generate harmonisedand comparable database at large scale and for calibration of hunting data into abundanceindices or population density. In addition, recommendations to select the methods to estimate the abundance or density and its implementations for ungulate populations are provided. How to increase the output quality provided by certain methods recognised as reliable (good accuracy and precision)and with the potential to be used for the validation and calibration ofother direct (i.e. based on observation of animals) or indirect (i.e. based on signs of animal activity) methods was recommended. Largely, the “counting” of large herbivores on a regional scale is often unfeasible, it can only be possible to accurately assess population status at local scale. We show that the habitat type plays a key role in the selection of the best method to determine density or relative abundance and that this is partially irrespective to species characteristics. A method that gives a density estimate rather than relative abundance, if possible, should be used. High‐quality hunting data statistics (collected at fine spatial resolution) have the highest availability and comparability potential across Europe, to give long‐term and large‐scale trends and should be used in predictive spatial modelling of wild ruminant relative abundance and density. Therefore, their standardized and harmonised collection is strongly recommended. On a local scale (e.g. management units), camera trapping is a method that can be conducted in different environmental conditions and at any time to collect robust data. In open areas, where camera trapping may require an excessive effort, we suggest using methods involving the direct detection of animals (vantage points, linear transects, block counts, random points). This should be carried out by correctly defining the study areas (for instance by means of distance sampling) and by estimating the repeatability of the results.

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Abstract

This guidance reviews the methods for estimating relative abundance and density in nine large European wild carnivore species, some representing relevant health concerns and provides insights on how to obtain reliable estimations by using those methods. On a local scale, the appropriate method should take into accountthe characteristics of the study area, the estimated survey efforts, the expected results (i.e. a measure of true density or just an index of abundance to monitor the trend in space and time) the level of accuracy and precision, and a proper design so to obtain a correct interpretation of the data. Among all methods, the camera trapping (CT) methods, especially those recently developed, are the most promising for the collection of robust data and can be conducted in a wide range of species, habitats, seasons and densities with minimal adjustments. Some recently developed CT methods do not require individual recognition of the animals and are a good compromise of cost, effort and accuracy. Linear transects, particularly Kilometric Abundance Index (KAI) is applicable for monitoring large regions. A large challenge is compiling and validating abundance data at different spatial scales. Based on ENETWILD initiative, we recommend developing a permanent network and a data platform to collect and share local density estimates, so as abundance in the EU, which would enable to validate predictions for larger areas by modelling. It would allow to identify gaps in the data on wild carnivores (including the species not assessed in the present report) and to focus on these areas for improving predictions. This platform must facilitate the reporting by wildlife policy makers and relevant stakeholders, but also citizen science initiatives. Also, there is need to improve the reliability of local density estimations by developing practical research on methods able to derive densities in untested species and situations, making the application of methods easier for local teams.

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2019

Abstract
By reviewing the different types of data targeted by the ENETWILD Wild Boar Data Collection Model (occurrence, hunting bag, abundance data) that have become available, an initial model could be built with occurrence data. A preliminary model analysis was performed to estimate the likely distribution of wild boar comparing the performance of a presence‐only model (bioclim) and presence‐background model (MaxEnt). Based on the results of this modelling, locations were identified, notably in Eastern Europe, where more data are required in order to produce more robust model projections of occurrence. This report also outlines the current state of available data collected by ENETWILD (occurrence, hunting bag and density) on wild boar and the development of a model framework that can be used to produce outputs on the yearly density distributions at high resolution of wild boar at a European scale.

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Abstract

After presenting preliminary models to estimate the habitat suitability for wild boar in MSs and neighbouring countries as a proxy for its relative abundance (i.e. the relative representation of a species in a particular ecosystem, a kind of proxy of the density) the ENETWILD consortium has developed further models for the estimation of wild boar abundance across this extent based on hunting yields (HY). This report therefore presents: i) updated maps of habitat suitability at 10×10 km resolution based on newly available data of wild boar occurrence together with new analysis to test the feasibility of performing such analysis at higher resolution (2×2 km); and, ii) a new model for predicting wild boar relative abundance, also at 10×10 km resolution, using hunting yields. The results of the occurrence model show that more occurrence data are required for specific locations in Eastern Europe in order to ensure robust model prediction of habitat suitability and consequently wild boar distribution. We used the hunting yields model to identify the environmental drivers of species abundance at European scale and fitted separate models for three regions (Southern, Western and Eastern Europe) to predict the distribution of wild boar at 10×10 km resolution. Our initial results highlighted some methodological issues relating to the statistical downscaling that should be taken into account to improve the reliability of the predictions. Whilst the spatial pattern in some areas was similar when comparing the predictions from both the occurrence and abundance models, in other regions there were marked discrepancies. To improve the models it is recommended to i) collect more occurrence data in the North‐Eastern region of Europe, in particular on survey effort; ii) combine regional and local hunting records to validate hunting yield predictions to higher spatial resolutions; and, iii) incorporate new environmental variables, especially those closely associated with wild boar abundance and distribution.

Abstract

In October 2018 the ENETWILD consortium created suitability maps based on available data on wild boar occurrence at 10 km square resolution and initial version of abundance models based on hunting statistics at NUTS3 and NUTS2 resolution, that were statistically downscaled for MSs to 10×10 km grid squares. This report presents updated suitability map for wild boar presence based on additional occurrence data and new algorithms, and new models based on high‐resolution hunting yield data for MSs and neighbouring countries. New environmental variables closely associated with wild boar abundance and distribution were also included. Our results showed no consensus for a single best occurrence model: out of those tested, both Maxent and random forest could be considered the best options depending on the choice of assessment metric. Predictions from these models notably disagreed in eastern Europe where data on wild boar occurrence are limited. Despite agreement among models, predictions in the south appeared over‐predicted, most likely due to a lack of contrasting absence data. Whilst there remain some methodological adjustments which could be tested, substantial improvement in the prediction from occurrence models relies on further collection of wild boar occurrence data in the east and complimentary data on survey effort in the south.The predictive performance of the hunting yield model was high. Although the incorporation of new data at higher spatial resolution markedly improved predictions, such data is still needed in some regions, ideally coupled with hunting effort, which would allow such estimates to be transformed into reliable densities. Comparison between predictions from the occurrence and hunting yield models showed they were statistically associated, but the strength of that relationship was dependent on the type of occurrence model and the bioregion. These findings are compatible with previous interpretations of the occurrence model, and highlight the relevance of obtaining more accurate data, especially from northern and eastern bioregions in Europe.

In October 2018 the ENETWILD consortium created suitability maps based on available data on wild boar occurrence at 10 km square resolution and initial version of abundance models based on hunting statistics at NUTS3 and NUTS2 resolution, that were statistically downscaled for MSs to 10×10 km grid squares. This report presents updated suitability map for wild boar presence based on additional occurrence data and new algorithms, and new models based on high‐resolution hunting yield data for MSs and neighbouring countries. New environmental variables closely associated with wild boar abundance and distribution were also included. Our results showed no consensus for a single best occurrence model: out of those tested, both Maxent and random forest could be considered the best options depending on the choice of assessment metric. Predictions from these models notably disagreed in eastern Europe where data on wild boar occurrence are limited. Despite agreement among models, predictions in the south appeared over‐predicted, most likely due to a lack of contrasting absence data. Whilst there remain some methodological adjustments which could be tested, substantial improvement in the prediction from occurrence models relies on further collection of wild boar occurrence data in the east and complimentary data on survey effort in the south.The predictive performance of the hunting yield model was high. Although the incorporation of new data at higher spatial resolution markedly improved predictions, such data is still needed in some regions, ideally coupled with hunting effort, which would allow such estimates to be transformed into reliable densities. Comparison between predictions from the occurrence and hunting yield models showed they were statistically associated, but the strength of that relationship was dependent on the type of occurrence model and the bioregion. These findings are compatible with previous interpretations of the occurrence model, and highlight the relevance of obtaining more accurate data, especially from northern and eastern bioregions in Europe.

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Abstract

Hunting statistics can be suitable to determine wild boar density estimates if a calibration with an accepted rigorous method is performed. Here, densities calculated from drive counts during collective drive hunting activities are compared against density values calculated by camera trapping using the random encounter method. For this purpose, we selected 10 study sites in Spain, from North to South representing a diversity of habitats, management and hunting traditions without artificial feeding, plus one study site in Czech Republic where artificial feeding was practiced. Density values estimated from both drive counts and camera trapping were strongly positively correlated (R2=0.84 and 0.87 for linear and non‐linear models, respectively) and showed a good agreement. Drive counts data might be therefore used as a density estimate to calibrate models for estimating density in large areas and potentially, to compare densities among areas. For these purposes, there is still the need to harmonise hunting data collection across Europe to make them usable at a large scale. Our results need to be confirmed across a wider number of European populations to provide valid geographical wild boar density predictions across Europe.

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The ENETWILD consortium implementedtheEFSA‐funded project “Wildlife: collecting and sharing data on wildlife populations, transmitting animal diseases agents”,whose main objective is to collect wild boardensity, hunting and occurrence dataand model species geographical distribution and abundance throughout Europe.This subject is of particular concern due to the continued advance of African swine fever (ASF). In May 2019,the ENETWILD consortiumorganised aworkshop for 30game biologists, animal health professionals, and experts from national huntingand forest authorities from 14 countries form North East Europe.The overall objectives of the workshop were to present milestones and achievements of the ENETWILD project,review different country frameworks forwild boar data collection and harmonization (hunting, density and occurrence data), as well as to review scientificmethods for determiningwild boar abundance and density, and train oncamera trapping and the random encounter method (REM).It was agreed thatwild boar abundance and densityestimates available in NorthEastern Europe are unreliable because most of them are not based on scientific methods. Hence, there is a need to implement a novel method for determining wild boar abundance and densitythat uses hunting bag statistics including measures of hunting effort and efficiency during collective drive hunts, compared against density values calculated using camera trapping and the random encounter method (REM). Several collaboratorsfrom Poland, Finland, Belarus, Russia, Lithuania have declared their willingness to participate in such pilot studies, and all agreed in improving data collection, including by means of citizen science

The ENETWILD consortium implementedtheEFSA‐funded project “Wildlife: collecting and sharing data on wildlife populations, transmitting animal diseases agents”, whose main objective is to collect wild boardensity, hunting and occurrence dataand model species geographical distribution and abundance throughout Europe.This subject is of particular concern due to the vastspread of African swine fever (ASF). In September 2019,the ENETWILD consortiumorganised aworkshop in Croatia for 27game biologists, animal health professionals, and experts from national huntingand forest authorities from 14 countriesfrom South East Europe.The overall objectives of the workshop were to present milestones and achievements of the ENETWILD project,to reviewthe framework forwild boar data collection and harmonization (hunting, density and occurrence data) of thedifferent countries,as well as to review scientificmethods for determiningwild boar abundance and density, and to train oncamera trapping and the random encounter method (REM).It was agreed thathunting bag data are currently the main source of information, although not always collected within a harmonized framework and rarely accompanied by a record of the hunting effort. Instead, wild boar abundance and densityestimates available in SouthEast Europe are unreliable because most of them are not based on scientific methods. Hence, there is a need to implement a novel method for determining wild boar abundance and densitythat uses hunting bag statistics including measures of hunting effort and efficiency during collective drive hunts, compared against density values calculated using camera trapping and the random encounter method (REM). Several collaborators have declared their willingness to participate in such pilot studies, and all agreed in improving data collection, including by means of citizen science.

2018

Abstract
The ENETWILD consortium (www.enetwild.com) has implemented an EFSA‐funded project whose main objective is to collect information and model the geographical distribution and abundance of wild boar throughout Europe. This is of particular concern owing to the spread of African swine fever from Eastern areas. In January 2018, ENETWILD organised discussion workshops for 70 experts in the field of the ecology, management and epidemiology of wild boar. Three workshops addressed the following questions: (1) what kind of data is needed to develop wild boar abundance maps?; (2) how can estimates of boar abundance be harmonised between regions?; and (3) how can the collection of wild boar distribution and abundance data be improved? In order to collect data on the presence/absence and abundance of wild boar obtained from different sources (administrations, hunters, naturalists and researchers), it is necessary to work on the generation, collection and processing of data in a harmonised manner, thus enabling the information to be comparable and used at a European level. The use of information on hunting statistics (number of animals hunted and hunting effort per surface unit) is particularly essential. The strategy is based on, firstly, collecting existing non‐harmonised wild boar data in the short‐term (occurrence and hunting statistics) by collecting the more accessible data. As a second step, ENETWILD distributed a questionnaire on how and where the data concerning hunting statistics are collected throughout the different Countries or regions in Europe. The objective of this questionnaire was to identify those places in which hunting statistics are still disaggregated (at the highest spatial resolution), with the purpose of standardising the means employed to collect hunting data in Europe. The following step consisted of the appropriate collection of data, using a data model and supported by a data‐sharing agreement.

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Abstract
The aim of this guidance is to assess the accuracy and reliability of the methods for estimation of density (i.e. population size per area unit) and relative abundance (i.e. relative representation of a species in a particular ecosystem, a kind of proxy of the density) of wild boar and to provide indications for calculating reliable and accurate estimates of those parameters using comparable methods. For this purpose eighteen methods were reviewed and evaluated. Since counting wild boar on a large regional scale is unfeasible, estimations of density and abundance are reliable only at local scale in specific habitats. Three methods (camera trapping, drive counts, and distance sampling with thermography) were recommended to estimate wild boar density on a local scale, and guidelines for their implementation was provided. In particular camera trapping is a method that can be conducted everywhere, irrespective of the habitat specificities and at any time to generate comparable data. Wild boar demographic data obtained by different methods cannot directly be combined by simple equations but spatial models are needed to determine abundance and predicted densities that are reliable at larger scales. On a large spatial scale and to describe long‐term trends, high quality hunting data statistics (collected on a fine spatial scale) have the highest availability and potential comparability potential across Europe, and these can be used in predictive spatial modelling of wild boar relative abundance and density. There is need for compiling and validating wild boar abundance data at different spatial scales: hunting bag data alone are not sufficient because a calibration with more accurate density estimation methods conducted at local scale is required. The latter are also required for evaluating predictive models for large areas and converting predicted relative abundances into densities.

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Abstract
This report provides a review of existing models for predicting the spatial distribution and abundance of wild boar at various scales (global, continental, national and regional) in order to inform the development of a new model to produce estimates of wild boar abundance at European level. The review identifies and discusses a range of models based on a wide variety of data types, corresponding to those targeted by the data collection model set by ENETwild, such as occurrence data (presence‐only, presence‐background and presence‐absence), hunting bag data and density data. The reviewed models are categorised in two main groups, the first based on occurrence data to predict a distribution of wild boar, and the second based on hunting bag, census and/or density data to directly model abundance. Owing to the diversity of methodologies, an ensemble modelling approach is here proposed for combining the outputs from a range of complimentary models and generating density estimates of wild boar at European scale. This would retain the flexibility necessary to utilise all available data whilst maintaining a robust output. An initial model has been outlined which uses occurrence data to generate wild boar distribution across Europe. The resulting suitability scores are related to available density estimates to establish a relationship, so the suitability map can be converted into a map of absolute density. In order to further utilize other types of data in this framework, the produced outputs of prediction of habitat suitability or presence/absence are used to underpin models based on available abundance data (hunting bag, census or density).

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Abstract
Heterogeneities in the wild boar data collection frameworks across Europe were analysed using questionnaires to explore comparability of hunting data in the short term and propose a common framework for future collection. Fifty‐seven respondents representing 32 countries covering more than 95% of European territory participated to the questionnaire. The most frequently recorded information in the official statistics included the quantity of animals shot per hunting ground and season (24 countries) and the size of the hunting (management) ground (21 countries). Georeferenced maps for the hunting grounds were collected (total or partial) for 20 countries. The least frequently recorded information was at the level of hunting events. We conclude that (i) sources of hunting statistics providing quantitative information on wild boar (and by extension, for other big game species) are lacking or are not harmonised across Europe, as well as incomplete, dispersed and difficult to compare; (ii) a feasible effort is needed to achieve harmonisation of data in a short time for the most basic statistics at the hunting ground level, and (iii) the coordination of the collection of hunting statistics must be achieved first at national and then at European level. The following is recommended: (i) countries should collect data at hunting ground level; (ii) efforts should be focused on data‐poor countries (e.g. Eastern Europe), and (iii) the data should be collected at the finest spatial and temporal resolution, i.e. at hunting event level. ENETWILD proposes the development of a robust and well‐informed data collection model as the basis for a common data collection framework. The present report identified some countries where, though the potential to share good quality data is present, the data collection promoted by ENETWILD has not succeeded so far (i. e. Eastern Europe). This highlights the need of further strategies to be developed so to encourage and support these countries to share hunting data.

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Reports by EFSA about African Swine Fever

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